本科毕业论文的摘要翻译成英文，求大神将下面的翻译下，不要在线翻译，要专业一些的，而且符合英语语法的

本实验以田间生长一个月左右的甘草幼嫩叶片为材料，探讨各种因素对甘草叶片原生质体分离的影响，这些因素包括酶解液组分、酶解时间、渗透压以及解离方式。通过实验发现:甘草幼嫩叶片在2.0%纤维素酶、0.5%果胶酶的酶解液中酶解效果最好，其产量为11.5×104个/g.FW；静置解离12h时所得到的原生质体最多，数量为11.6×104个/g.FW;渗透压为0.35M的酶解液对于甘草幼嫩叶片原生质体分离的效果最佳，原生质体产量为11.1×104个/g.FW;采用静置加缓慢震荡酶解所获得的原生质体质量最佳，产量最高，为12.5×104个/g.FW。本研究为甘草的原生质体培养及体细胞杂交奠定了基础。

In this experiment,field growthlicoricetender leavesa monthas materials,effects of various factors onseparationofliquoriceleaf protoplasts,these factors include theenzyme solutioncomponents,enzymolysis time,osmotic pressure anddissociation.Through the experiment discovered:young leaves ofGlycyrrhizasolutionenzymolysiseffect is the bestin 2% cellulase,pectinase0.5%,its yieldis 11.5 ×104 /g.FW;protoplastwasstandingby thedissociation of12hmost,amount to 11.6× 104 /g.FW;osmotic pressure of 0.35M enzyme solution on the separation in the best licorice leaf protoplast,protoplast yield was 11.1 × 104 /g.FW; with static and slowly oscillating enzyme obtained by protoplast the best quality, the highest yield, 12.5 × 104/g.FW. This study lays a foundation for protoplast culture andsomatic hybridization oflicorice.

只能做到这些了⊙﹏⊙

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