第1个回答 2011-02-02
Keywords: heavy metal ions, pollution, chlorophyll, arabidopsis
Keyword: heavy metal, Arabidopsis thaliana, chlorophyll, leaves something
First part: background: tianjin, heavy metal pollution and on chlorophyll influence, arabidopsis introduction
The second part: the implementation of part: planting, sewage preparation, processing, chlorophyll content determination
Part 3: summary: under heavy metal ions pollution arabidopsis chlorophyll content changes and topic significance
4 choose arabidopsis as test materials
Biologists through for the selected biological species of scientific research, used to discover some has the universal law life phenomenon, at this time, this was selected species is model organisms. Arab thaliana is a kind of cruciferous plants, widely used in plant genetics and molecular biology, and developmental biology research, has become a kind of typical model plant, its reason mainly based on the plant has the following features:
(1) plant morphological individual small, height only about 30cm, 1 cup can be planted several tree,
(2) the growth cycle quickly, each generation time is short, from sowing to harvest seed typically require 6 weeks or so,
(3) seeds, bunches of each generation can produce thousands of seed;
(4) morphological characteristics, strong vitality, simple to use common medium can be artificial cultivation,
(5) genome small, only 5 of chromosomes.
The second part: the implementation: testing containing different concentration sewage with heavy metal ions of arabidopsis chlorophyll content effects for sewage disposal, provides the basis. (inquires Pb and Cd, metal ion Cu in water allows content)
[equipment and reagents.
Arabidopsis seeds, flower POTS, nutritional soil, spectrophotometer, centrifuge, balance, scissors, grind bowls, funnel, pipette, beaker, acetone, quartz sand, Cd, Pb (NO2) 2) 2, Cu (NO2 (NO2) 2, distilled water
1 arabidopsis planting:
Choose good water imbibition, soil soft nutritional soil as arabidopsis planting soil. In diameter 9cm flower, every laver sowing 20-30 star. After sowing in flowerpot on the hood film, give a growth of plant offers a wet environment. In on the fifth day, about in the fourth deried from weeks after the third piece of cotyledons grow and began to experiment.
2 containing heavy metal ions sewage compound
Compound containing Cd (NO3) 2, Pb (NO3) 2, Cu (NO3) 2 solution, respectively satisfy its contain Cd2, Pb2 + + Cu2 + ions concentration, for 5mg/L, 25mg/L, 50mg/L, 100mg/L
(process oneself write detailed point, translated into English.
3 chlorophyll content change: adopt different concentrations of aqueous arabidopsis
To grow in arabidopsis flower adds contains three kinds of different heavy metal ion concentration for 5mg/L, 25mg/L, 50mg/L, 100mg/L sewage 30mL, arabidopsis of low concentration of heavy metal ion pollution have patience, but as time increases, poisons action strengthen gradually, chlorophyll content decreased, growth is restrained. The experimental results showed that under different concentrations heavy metal processing, arabidopsis chlorophyll content in the first days there were no changes, the next three days chlorophyll content changes (down) compared with control significant level, especially for the first four days, chlorophyll content with cadmium concentration of aqueous increase significantly.
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第2个回答 2011-02-03
First part: background: tianjin, heavy metal pollution and on chlorophyll influence, arabidopsis introduction
The second part: the implementation of part: planting, sewage preparation, processing, chlorophyll content determination
Part 3: summary: under heavy metal ions pollution arabidopsis chlorophyll content changes and topic significance
4 choose arabidopsis as test materials
Biologists through for the selected biological species of scientific research, used to discover some has the universal law life phenomenon, at this time, this was selected species is model organisms. Arab thaliana is a kind of cruciferous plants, widely used in plant genetics and molecular biology, and developmental biology research, has become a kind of typical model plant, its reason mainly based on the plant has the following features:
(1) plant morphological individual small, height only about 30cm, 1 cup can be planted several tree,
(2) the growth cycle quickly, each generation time is short, from sowing to harvest seed typically require 6 weeks or so,
(3) seeds, bunches of each generation can produce thousands of seed;
(4) morphological characteristics, strong vitality, simple to use common medium can be artificial cultivation,
(5) genome small, only 5 of chromosomes.
The second part: the implementation: testing containing different concentration sewage with heavy metal ions of arabidopsis chlorophyll content effects for sewage disposal, provides the basis. (inquires Pb and Cd, metal ion Cu in water allows content)
[equipment and reagents.
Arabidopsis seeds, flower POTS, nutritional soil, spectrophotometer, centrifuge, balance, scissors, grind bowls, funnel, pipette, beaker, acetone, quartz sand, Cd, Pb (NO2) 2) 2, Cu (NO2 (NO2) 2, distilled water
1 arabidopsis planting:
Choose good water imbibition, soil soft nutritional soil as arabidopsis planting soil. In diameter 9cm flower, every laver sowing 20-30 star. After sowing in flowerpot on the hood film, give a growth of plant offers a wet environment. In on the fifth day, about in the fourth deried from weeks after the third piece of cotyledons grow and began to experiment.
2 containing heavy metal ions sewage compound
Compound containing Cd (NO3) 2, Pb (NO3) 2, Cu (NO3) 2 solution, respectively satisfy its contain Cd2, Pb2 + + Cu2 + ions concentration, for 5mg/L, 25mg/L, 50mg/L, 100mg/L
(process oneself write detailed point, translated into English.
3 chlorophyll content change: adopt different concentrations of aqueous arabidopsis
To grow in arabidopsis flower adds contains three kinds of different heavy metal ion concentration for 5mg/L, 25mg/L, 50mg/L, 100mg/L sewage 30mL, arabidopsis of low concentration of heavy metal ion pollution have patience, but as time increases, poisons action strengthen gradually, chlorophyll content decreased, growth is restrained. The experimental results showed that under different concentrations heavy metal processing, arabidopsis chlorophyll content in the first days there were no changes, the next three days chlorophyll content changes (down) compared with control significant level, especially for the first four days, chlorophyll content with cadmium concentration of aqueous increase significantly.
第3个回答 2011-02-02
Part I: Background: Tianjin, heavy metal pollution and the impact of chlorophyll, Arabidopsis Introduction
Part II: Implementation part: planting, water preparation, treatment, determination of chlorophyll
Part III: Summary: Arabidopsis thaliana under heavy metal pollution, changes in chlorophyll content and the subject of significance
4 selected as the test material in Arabidopsis
Biologists selected species through scientific research, to reveal a universal law of life phenomena, this time, this species is that the selected model organisms. Is a cruciferous plant Arabidopsis thaliana, widely used in plant genetics, developmental biology and molecular biology, has become a typical pattern of plants, mainly due to the plant based on the following features:
(1) plant morphology small size, height of only 30cm or so, a good cup for planting trees;
(2) fast growth cycle, every generation time is short, generally from seed sowing to harvest only about 6 weeks;
(3) seed number per plant per generation can produce thousands of seeds;
(4) morphological characteristics of simple, vitality, can be made with ordinary artificial culture medium;
(5) genome is small, only 5 pairs of chromosomes.
Part II: Implementation: Testing of heavy metal ions with different concentrations of sewage on the chlorophyll content of Arabidopsis thaliana, provide the basis for wastewater treatment. (Query metal ions Cd, Pb and Cu content in the water allowed)
】 【Equipment and reagents
Arabidopsis seeds, flower pots, nourishment soil, spectrophotometer, centrifuges, scales, scissors, mortar, funnel, pipette, beaker, acetone, quartz sand, Cd (NO2) 2, Pb (NO2) 2, Cu (NO2) 2, distilled water
An Arabidopsis plant:
Select absorbent, soft soil nutritive soil as planting soil in Arabidopsis. 9cm in diameter pot, the pot planting 20-30 teeth. After sowing in pots on the cast film, the growth of a plant to provide a moist environment. Sprouting in the fifth day, when about to grow in the fourth week after the third film leaves began to experiment.
2, preparation of sewage containing heavy metal ions
Preparation containing Cd (NO3) 2, Pb (NO3) 2, Cu (NO3) 2 solution, respectively, to contain Cd2 +, Pb2 +, Cu2 + ion concentration of 5mg / L, 25mg / L, 50mg / L, 100mg / L
(Written in some detail the process of their translation into English.)
3 changes of chlorophyll content: solution treated with different concentrations of Arabidopsis
To the cultivation of Arabidopsis thaliana were added to pots containing three different concentrations of heavy metals 5mg / L, 25mg / L, 50mg / L, 100mg / L of sewage 30mL, Arabidopsis heavy metal ion contamination of low concentration to a certain extent patience, but as time increases, the toxic effects gradually increased, decreased chlorophyll content, growth was inhibited. The results showed that treatment with different concentrations of heavy metals, the Arabidopsis chlorophyll content did not change on day 1, followed by 3 days the chlorophyll content of the change (decrease) compared with the control group reached a significant level, especially the first 4 days , chlorophyll content increased with increasing concentration of Cd decreased significantly.本回答被提问者采纳
第4个回答 2011-02-02
First part: background: tianjin, heavy metal pollution and on chlorophyll influence, arabidopsis introduction
The second part: the implementation of part: planting, sewage preparation, processing, chlorophyll content determination
Part 3: summary: under heavy metal ions pollution arabidopsis chlorophyll content changes and topic significance
4 choose arabidopsis as test materials
Biologists through for the selected biological species of scientific research, used to discover some has the universal law life phenomenon, at this time, this was selected species is model organisms. Arab thaliana is a kind of cruciferous plants, widely used in plant genetics and molecular biology, and developmental biology research, has become a kind of typical model plant, its reason mainly based on the plant has the following features:
(1) plant morphological individual small, height only about 30cm, 1 cup ...
第5个回答 2011-02-02
这个真是相当的学术和专业啊,不好意思,爱莫能助.